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KMID : 0384119930130030429
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 3 p.429 ~ p.436
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Evaluation of a Commercial Microdilution(ATB STREP) System for Detection of High-Level Aminoglycoside Resistance in Enterococci
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Abstract
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The ATB STREP system(API system, Bio Merieux, France) was compared with agar dilution screen plates as a reference method for the detection of high-level aminoglycoside resistance in 112 enterococcal isolates, including 72 E. faecalis, 17 E.
faecium, 10
E. raffinosus and 8 E. avium. The strains showing no high-level resistance on the ATB STREP system and the specificity of ATB STREP system was 100%. When tested on agar dilution plates, 75(67.0%), 81(72.3%) and 55(49.1%) strains showed high-level
resistance to gentamicin(500μg/ml), kanamycin(500μg/ml, 2000μg/ml) and streptomycin (1000μg/ml, 2000μg/ml), respectively. The number of enterococcal strains showing high-level resistance to gentamicin, kanamycin and streptomycin on the ATB
STREP
system were 71, 81 and 40 respectively, resulting in sensitivities of 94.7%, 100% and 72.2%, and the discordant data were observed only in E. faecalis. We found that prolonged incubation of ATB STREP system to 48 hours or visual reading (for the
detection of light growth) at 24 hours could improve the sensitivity of ATB STREP system for detection of high-level resistance to gentamicin or streptomycin in E. faecalis. These results suggest that 24 hour visual reading or prolonged
incubation
to 48
hour is needed for routine use of the ATB STREP system.
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KEYWORD
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